RESUMO
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Criptosporidiose/diagnóstico , Ensaio de Imunoadsorção Enzimática/normas , Fezes/parasitologia , Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Animais , Estudos de Casos e Controles , Cryptosporidium/imunologia , Cryptosporidium/isolamento & purificação , Humanos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100 percent, with a specificity of 96 percent; positive and negative predictive values were 89 percent and 100 percent, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection.